Workflow de clonage d'ADN Trucs de PCR et trucs de clonage dMADN par Simon Roy, Ph.D Cette page sera mise à jour plusieurs fois. Il vous serait utile de la consulter dans le futur afin d’obtenir des mises à jour sur mes protocoles. ”Bookmark” ...
Overlap Extension PCR Protocol PCR Protocol for DNA assembly by PCR extension of overlapping DNA fragments. PCR overlap extension is useful for DNA cloning and site-directed mutagenesis. Here, you will find 2 different protocols a standard protocol for performing...
Fast & Steep PCR Protocol Fast PCR Protocol for Cloning and Site-Directed Mutagenesis Fast & Steep PCR can be used for quick DNA amplification from plasmid DNA, purified DNA fragments and assembly PCR by extension of overlapping DNA fragments. Do it with FLYMain...
Fast Mutagenesis Protocol with FM111 Site-Directed Mutagenesis using WVA and partially overlapping primers Get the Fast Mutagenesis KitGo to the Main SDM page ; TransBionovo's Fast Mutagenesis Protocol Advantages Semi-exponential amplification PCR. Both primers...
QuickChange Site-Directed Mutagenesis Protocol Site-Directed Mutagenesis using WVA and complementary primers Go to the Main SDM pageGet inspired by our PCR Success Stories ; QuickChange Site-Directed Mutagenesis Advantages Both primers contain the mutation. In vitro...
KLD Site-Directed Mutagenesis Protocol Site-Directed Mutagenesis using WVA, back-to-back primers and KLD Build your own KLD Mutagenesis KitGo to the Main SDM Page ; KLD Site-Directed Mutagenesis Protocol using Back-to-Back Primers Advantages of KLD Site-Directed...
