KD Plus DNA Polymerase (Ultra-High-Fidelity)
TransStart® KD Plus DNA Polymerase is a genetically modified ultra-high fidelity DNA polymerase in hotstart formulation. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. It also benefits from the TransStart® double Hot start technology.
NOTE from Civic Bioscience : In our hands, KD Plus is the High-Fidelity DNA Polymerase of choice for AT-rich PCR that often requires an extension temperature of 62-68°C.
Advantages of TransStart® KD Plus DNA Polymerase
- PCR product carries blunt ends, which can be directly cloned into pEASY®-Blunt vectors.
- It’s moderate amplification speed (1 kb/min) allows for better amplification of GC-rich and AT-rich templates
- Its ultra high proofreading capacity makes this polymerase ideal for cloning, library amplification and other application requiring excellent fidelity.
- Amplification of genomic DNA fragment up to 15 kb.
- Amplification of plasmid DNA fragment up to 20 kb.
- Available with or without 2.5 mM dNTPs (1 ml) for additional convenience.
Manual and datasheet
Additional information
| Format | 6 x 200 u, 6 x 200 u + dNTPs, 100 u, 100 u + dNTPs, 200 u, 200 u + dNTPs |
|---|---|
| Supplier |
Reviews for TransStart KD Plus DNA Polymerase (Ultra-HiFi) - AP301
4 reviews for TransStart KD Plus DNA Polymerase (Ultra-HiFi) - AP301
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srcivicbio –
User comment and results submitted by email from a customer at Université de Montréal. For privacy reasons, the amplified genes are not specified.
Voici le gel dont je t’avais parlé. La piste 8 est la plus révélatrice. Tu peux la comparer aux autres enzymes 6,7 et 9.
>Gene : 8.4kbp
GC% : 70%
Pistes :
1. MW
2.à 5. c’était des tests avec une ADN de départ avec GC 30%, même enzymes mais différents primers.
6. FastPfu Fly
7. FastPfu
8. KD Plus
9. Q5 HS
Simon Roy (verified owner) –
Comment received by email from the University of British Columbia:
I succeeded amplifying a 2.5k fragment with KD PLUS and trying to amplify another fragment. Before that, I tried FastPfu but it seems like KD Plus is optimal for my case.
Dominique Lévesque (verified owner) –
one of the most powerful polymerase I ever used. I recommend!!! five stars!!!
Phil Roche –
Using the Pre-mix Kdplus we’ve been very happy with it’s performance in GC rich amplifications. Good yield with reliable, high fidelity product.