TransStart FastPfu FLY DNA Polymerase (Ultra High-Fidelity) - AP231
This product has been replaced by King Kong Pfu Ultra-High Fidelity DNA Polymerase CB-KKP-011
$258.00 – $1,321.00 CAD
0 in stock (expect 7-21 days for delivery)
FastPfu FLY DNA Polymerase
TransStart FastPfu Fly DNA Polymerase is a hotstart, ultra-high fidelity and high-processivity DNA Polymerase. It consists in a modified DNA Polymerase, increasing its affinity for dsDNA and enhancing processivity and other caracteristics. Its fidelity is 108 times higher than EasyTaq DNA polymerase making it the DNA Polymerase with the highest fidelity on the market. In addition, its extension rate reaches 6 kb/min. Compared with FastPfu DNA Polymerase, The FLY version of FastPfu DNA High-Fidelity Polymerase has a faster extension rate, higher fidelity, and greater amplification efficiency over regular FastPfu, making FastPfu FLY the best performing DNA polymerase for ultra low non-specific amplification from complex and unpurified gDNA samples, as well as other difficult PCRs targeting GC-rich or AT-rich DNA sequences. FastPfu FLY is available for NGS library prep amplification.
- Learn all about FLY
- GC-rich PCR competitor comparison.
- GC-rich PCR competitor comparison - PCR Success Story #21
Advantages of FastPfu Fly DNA Polymerase AP231
- Fidelity is 108 times higher than EasyTaq DNA Polymerase.
- TransStart HotStart : Extremely specific amplification.
- The extension rate is about 2-6 kb/min.
- PCR products can be directly cloned into pEASY®-Blunt vectors.
- Amplification of genomic DNA fragment up to 15 kb.
- Amplification of plasmid DNA fragment up to 20 kb.
- Available with or without 2.5 mM dNTPs for additional convenience !
Manual and datasheet
This product has been replaced by King Kong Pfu Ultra-High Fidelity DNA Polymerase
Publications
- Kevin Neil, Nancy Allard, David Jordan, Sébastien Rodrigue. Assembly of large mobilizable genetic cargo by double recombinase operated insertion of DNA (DROID). Plasmid..Volume 104, 102419. July 2019 (PDF download)
Additional information
| Format | 6 x 500u, 6 x 500u + dNTPs, 250u, 250u + dNTPs, 500u, 500u + dNTPs |
|---|---|
| Supplier |
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6 reviews for TransStart FastPfu FLY DNA Polymerase (Ultra High-Fidelity) - AP231
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srcivicbio –
Customer review submitted by email.
Agarose gel electrophoresis after site-directed mutagenesis ‘Quickchange’ protocol.
-Wells 2 to 4: PastPfu FLY TGAP231
-Wells 5 to 7: KD Plus TGAP301
-Wells 8-9: Pfu Ultra (Agilent)
Bonjour Simon,
J’aimerais savoir le prix de ta polymerase TransStart FastPfu FLY DNA Polymerase. Je l’ai essayée et elle va très bien. Je vais essayer de convaincre mon patron pour en avoir. Je ne peux pas te dévoiler le vecteur utilisé pour cause de confidentialité. Merci!
Simon Roy (verified owner) –
Commentaire reçu par courriel:
• Utilisable comme la Veraseq
• Pas chère
• Utilisable diluée jusqu’à 1/8X
• Amplification de long fragments (13kb+)
Joelle
PL –
Salut Simon,
La FLY… elle fly en Taqarnac!
Et les colonnes gel/pcr purification fonctionnent très bien.
Tu auras aussi officiellement des acheteurs pour tes colonnes pour 2 labos (ils attendaientt les résultats de mes séquençages pour passer une commande de colonnes chez vous)
Patrick –
In my own hands, you can pick up more targets if you use 68 as the extension temperature.
This will favor the amplification of less stable molecules including AT rich sequences.
Note that this may disfavor amplification of gc rich targets.
If used with PCR stimulant, 68 behave similarly to 72 without PCR stimulant.
I use 68 in 95% of my reactions
If GC rich and very stubborn. Try 75 for extension.
Excellent enzyme
Simon Roy (verified owner) –
Commentaire reçu par email le 2018/05/15:
Merci pour tes protocoles. J’ai utilisé le Fast & Steep PCR à quelques reprises et je suis vraiment stupéfait des résultats obtenus en si peu de temps de PCR avec la FLY!
Pat –
Clonage Fast and Clean. Gel d’agarose pour gel extraction du Vecteur digéré et des fragment Left et Right avant le clonage par assembly CU101. Go Fly!