TransNGS Stranded RNA-Seq Library Prep Kit for Illumina - KP301
507.00$ – 5,745.00$CAD
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Stranded RNA Seq Library Prep Kit for Illumina
TransNGS Stranded RNA-Seq Library Prep Kit for Illumina is designed to prepare Stranded RNA libraries compatible with the Illumina® sequencers. Differently from conventional stranded RNA seq library prep kits, the TransNGS Stranded RNA seq kit generates cDNA using random hexamer with a 3’-Adaptor. Subsequently, the 5’-Adaptor is ligated at the 3’-end of the first-strand cDNA, bypassing the need for 2nd strand synthesis and digestion. Then, end-repair and dA-tailing are performed. As a result, only information from the 1st strand cDNA is preserved. This kit is tested and promised to provide stable and reproducible cDNA libraries, by using either total RNA samples, rRNA-depleted samples, mRNA purified samples or lncRNA samples.
Highlights
- User-friendly and fast with minimal hands-on time
- High efficiency with minimal amount of input DNA
- Operation is simple and fast
Applications
- Gene expression analysis
- Single–nucleotide variation (SNV) discovery
- Splice junction and gene fusion identification
- Whole transcriptome analysis
- Characterization of non-polyadenylated RNAs, including non-coding and immature RNAs
Kit Contents
Components | KP301-01 (6rxns) | KP301-02 (24rxns) | KP301-03 (96rxns) |
2×RNA-Seq RT Reaction Mix | 60 μl | 240 μl | 4×240 μl |
RNA-Seq RT Enzymes Mix | 6 μl | 24 μl | 4×24 μl |
RNA Digestion Mix | 6 μl | 24 μl | 4×24 μl |
cDNA Binding Buffer | 180 μl | 720 μl | 4×720 μl |
5′-Adaptor | 6 μl | 24 μl | 96 μl |
2×RNA-Seq Ligation Mix | 150 μl | 600 μl | 4×600 μl |
RNA-Seq Ligase | 30 μl | 120 μl | 480 μl |
RNA Ligation Stop Buffer | 200 μl | 800 μl | 4×800 μl |
TransNGSTM Library Amplification SuperMix | 150 μl | 600 μl | 4×600 μl |
Library Elution Buffer (LEB) | 1.2 ml | 5 ml | 4×5 ml |
RNase-free Water | 1 ml | 1 ml | 5 ml |
Cat. No. KP301
Storage: at -20°C for one year
RNA Input Recommendations
Starting Materials: 10 ng – 1 μg total RNA treated with rRNA depletion, 0.1 μg – 4 μg total RNA treated with mRNA purification and lncRNA.
Requirements: high quality RNA, which is recommended to use an Agilent 2100 Bioanalyzer to analyze the total RNA. The RIN (RNA integrity number) value should be ≥ 7.0. Using degraded total RNA for library construction will lead to in 3’ bias in RNA-seq. The ratio of OD260/OD280 should be between 1.8 and 2.1.
Reagents not included in the kit
- 80% ethanol (freshly prepared);
- DNA size selection beads like MagicPure Size Selection DNA Beads (TransGen, EC401);
- Index Primers : TransNGS Single Index Primers Kit for Illumina® (TransGen, KI201-KI221) or Dual Index Primers Kit for Illumina® (TransGen, KI231).
Workflow for Stranded RNA library prep
Manual & Datasheet
KP301 - TransNGS Stranded RNA-Seq Library Prep Kit for Illumina - 2018-11-07
Additional information
Format | 6 rx, 24 rx, 96 rx |
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