TransDB3.1 (ccdB resistant) Competent Cell - CD531

TransDB3.1 Chemically Competent Cell is specifically designed for chemical transformation of DNA. This strain contains the gyrA462 gene which provides resistance to the toxic effects of the ccdB gene. TransDB3.1 Chemically Competent Cell can be used for transformation and propagation of plasmid containing the ccdB gene.

F- gyrA462 endA1 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) supE44 ara-14 galK2 lacY1 proA2 rpsL20(SmR) xyl-5 λ-leu mtl1

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Genotype definitions (if applicable to this product)

Genotype	Description
ara-14	Blocks arabinose catabolism
argF	Ornithine carbamoyltransferase mutation blocks ability to use arginine
dam/dcm	Abolishes endogenous adenine methylation at GATC sequences (dam) or cytosine methylation at CCWGG sequences (dcm). Used to propagate DNA for cleavage with certain restriction enzymes (e.g. Ava II, Bcl I)
DE3	Lysogen that encodes T7 RNA polymerase. Used to induce expression in T7-driven expression systems
endA	endA Mutation in the non-specific endonuclease Endonuclease I; eliminates non-specific endonuclease activity, resulting in improved plasmid preps
F´	A self-transmissible, low-copy plasmid used for the generation of single-stranded DNA when infected with M13 phage; may contain a resistance marker to allow maintenance and will often carry the lacI and lacZ∆M15 genotypes
galK	Galactokinase mutation blocks catabolism of galactose—cells that are galK minus grow in the presence of galactose as the sole carbon source
galU	Glucose-1-phosphate uridylyltransferase mutation blocks ability to use galactose—cells that are galU minus can grow on media that contains galactose as the sole carbon source
gyrA96	DNA gyrase mutant produces resistance to nalidixic acid
hsd	Mutations in the system of methylation and restriction that allow E. coli to recognize DNA as foreign. The hsd genotype allows efficient transformation of DNA generated from PCR reactions *hsdR–eliminates restriction of unmethylated EcoK I sites. (1) **hs
lacI	Encodes the lac repressor that controls expression from promoters that carry the lac operator; IPTG binds the lac repressor and derepresses the promoter; often used when performing blue/white screening or to control expression of recombinant genes
lacY1	Blocks use of lactose via β-D-galactosidase mutant
lacZ	β-D-galactosidase gene; mutations yield colorless (vs. blue) colonies in the presence of X-gal
lacZ∆M15	Element required for β-galactosidase complementation when plated on X-gal; used in blue/white screening of recombinants; usually carried on the lambdoid prophage φ80 or F´
leuB	Requires leucine for growth on minimal media via β-isopropyl malate dehydrogenase mutation
lon	lon Deficiency in the Lon ATPase-dependent protease; decreases the degradation of recombinant proteins; all B strains carry this mutation
mcrA, mcrBC,or mrr	Mutations that allow methylated DNA to not be recognized as foreign; this genotype is necessary when cloning genomic DNA or methylated cDNA
nupG	Mutation for the transport of nucleosides
ompT	Indicates that the E. coli lack an outer membrane protease—reduces degradation of heterologous the strains and recovery of intact recombinant proteins is improved in ompT minus strains
P3	A 60-kb low-copy plasmid that carries the ampicillin and tetracycline resistance genes with amber mutations; used predominantly for selection of supF-containing plasmids; carries the kanamycin resistance gene for selection
pLys	pLys Plasmid that encodes T7 lysozyme; used to reduce basal expression in T7-driven expression systems by inhibiting basal levels of T7 RNA polymerase
proAB	proAB Requires proline for growth on minimal media
recA	Mutation in a gene responsible for general recombination of DNA; particularly desirable when cloning genes with direct repeats
relA	RNA is synthesized in absence of protein synthesis (relaxed phenotype) relA locus regulates the coupling between transcription and translation. In the wild type, limiting amino acid concentrations results in the shutdown of RNA synthesis (also known as th
rpsL	Confers resistance to streptomycin (this makes a mutant ribosomal protein, small subunit, the target of the drug)
supE,F	tRNA glutamine suppressor of amber (supE)(UAG) or tyrosine (supF)
thi-1	Requires thiamine for growth on minimal media
Tn10	Confers tetracycline resistance via a transposon
tonA	Confers resistance to the lytic bacteriophage T1, T5 and f80
traD, D36	Prevents transfer of F’ episome via transfer factor mutation
tsx	Confers resistance to phage T6 and colicin K
xyl-5	Blocks catabolism of xylose