TransB(DE3) Chemically Competent Cell - CD811

TransB(DE3) Chemically Competent Cell

TransB(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to kanamycin (Kan^R) and tetracycline (Tet^R) and permits a transformation efficiency of over 10⁷ cfu/μg DNA (tested by pUC19 plasmid DNA). Control Plasmid I (Amp⁺) is used to detect cell expression function, the size of expressed protein is about 25 kDa.

Features of TransB DE3 cells

• Transformation efficiency: >10⁷ cfu/μg (pUC19 DNA).
• Kan^R and Tet^R.
• Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.
• Control plasmid I (Amp⁺) is used for detection of expression function of cell. The protein size is about 25 kDa.

Genotype

F- omp T hsd SB( r B- mB-) gal dcm lac Y1 ahpC (DE3)gor 522::Tn10 trxB (KanR，TetR).

Complete Expression Competent Cell comparison and selection chart

Manual and Datasheet

CD811 TransB(DE3) Chemically Competent Cell