PGE1 (Prostaglandin E1) ELISA Kit - E-EL-0052

PGE1 (Prostaglandin E1) ELISA Kit

The PGE1 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with PGE1. During the reaction, PGE1 in the sample or standard competes with a fixed amount of PGE1 on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to PGE1. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: PGE1

Abbreviation : Prostaglandin E1 ,PGE1,alprostadil

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 46,875

Detection Range : 78.125-5000 pg/mL

More information about this PGE1 (Prostaglandin E1) ELISA Kit from Elabscience