pEASY®-T5 Zero Cloning Kit - CT501

pEASY®-T5 Zero T/A TOPO Cloning Kit

pEASY®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment containing 3’A-overhangs) disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. The vector is also designed for T3 and T7 RNA polymerases in vitro transcription. T7 RNA polymerase-mediated transcription can be performed using our T7 High-Efficiency Transcription Kit. Visit our DNA Polymerase selection chart to find the best DNA polymerase suitable for T/A cloning.

Features of the pEASY®-T5 Zero Cloning Kit

• 5 minutes fast ligation of Taq-amplified (3’A overhangs) PCR products.
• High cloning efficiency. Positive clones up to 100%.
• No blue/white selection needed.
• Suitable for short and large fragment cloning.
• Kanamycin and Ampicillin resistance genes for choice of selection.
• M13 forward primer and M13 reverse primer for sequencing.
• T3 promoter and T7 promoter for in vitro transcription.
• Includes Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>10⁹ cfu/μg pUC19 DNA) and fast growth.

Datasheet and manual

CT501 pEASY ®-T5 Zero Cloning Kit

pEASY-T5 Zero Snapgene (linearized)

T5 Zero Complete sequence