pEASY-Basic Seamless Cloning and DNA Assembly Kit - CU201
Custom kit available for only 272 $ when combined with any High-Fidelity DNA Polymerase
Basic Seamless Cloning and DNA Assembly
The pEASY-Basic Seamless Cloning and Assembly Kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments.
Features of the pEASY-Basic CU201
- Fast assembly and fast cloning: 15 minutes
- Easy: regardless of restriction enzyme sites of inserts, and elimination of restriction digestion of inserts
- High efficiency: up to 95% positive clones
- Seamless Assembly: no extra sequences introduced
- Includes High-Efficiency Trans1-T1 Phage Chemically Competent Cells.
Component | CU201-02 | CU201-03 |
2×Basic Assembly Mix | 100 μl | 6×100 μl |
Trans1-T1 Phage Resistant Chemically Competent Cell | 10×100 μl | 30×100 μl |
Linearized pUC19 Control Vector (10 ng/μl) | 6 μl | 18 μl |
Control Insert (1 kb,20 ng/μl) | 6 μl | 18 μl |
Datasheet and manual
Additional information
Format | 20 rx, 60 rx |
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