Myelin Basic Protein, MBP Antibody - CPCA-MBP

Encor Chicken Polyclonal to Myelin Basic Protein, MBP

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (1). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (2). In this case the proteins are referred to as Golli-MBP proteins and result from the use of 3 exons not utilized in the nervous system, resulting in proteins with unique N-terminal regions.
In the central nervous system there are four different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 20.5, 18.5, and 17.2 kDa in humans. The single gene of rodents also produces 4 different proteins, but of slightly different sizes, 21.5, 18.5, 17 and 14 kDa. Sequence alignments of both human and rodent isotypes can be downloaded from here. We have found that our chicken antibody stains all four transcripts on blots with equivalent intensity. We also market two good mouse monoclonal antibodies to MBP, MCA-7G7 and MCA-7D2. The HGNC name for this protein is MBP.

Antibody was raised in chicken against purified myelin basic protein isolated from cow nerve. This antibody is an IgY preparation, with total protein content about 30 mg/mL.

HGNC name(s) : MBP

Host : Chicken

Clonality : Polyclonal

ID : EnCor Biotechnology Myelin Basic Protein, MBP MBP

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgY

Conjugation : none

Immunogen : Purified bovine

Mass of detected protein : 14, 17, 18.5, and 21.5 kDa

Uniprot ID : P02686

KGNC name : MBP

RRID # : AB_2572352

Purification : Concentrated IgY preparation

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:10 000. IF/ICC and IHC: 1:1 000.

Datasheet

Link to on EnCor’s website

References :

1. Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P. Basic A1 protein of the myelin membrane. The complete amino acid sequence. J. Biol. Chem. 246:5770-5784 (1971).

2. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861 (2002).