IP3 (Inositol Triphosphate) ELISA Kit - E-EL-0059

IP3 (Inositol Triphosphate) ELISA Kit

The IP3 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with IP3. During the reaction, IP3 in the sample or standard competes with a fixed amount of IP3 on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to IP3. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: IP3

Abbreviation : Inositol trisphosphate , inositol 1,4,5-trisphosphate ,triphosphoinositol, InsP3,Ins3P,IP3,

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 9,375

Detection Range : 15.625-1000 pg/mL

More information about this IP3 (Inositol Triphosphate) ELISA Kit from Elabscience