Human EGF (Epidermal Growth Factor) ELISA Kit - E-EL-H0059

Human EGF (Epidermal Growth Factor) ELISA Kit

The Human EGF ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EGF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for EGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of EGF. You can calculate the concentration of EGF in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Epidermal Growth Factor

Abbreviation : EGF

Uniprot ID : P01133

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 2.344 pg/ml

Detection Range : 3.906 - 250 pg/ml

Manual & Datasheet

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