Fast Mutagenesis System - FM111

Fast Mutagenesis System

Design for the Fast Mutagenesis System

• Primers anneal to the DNA template, mutant strands are synthesized with TransStart® FastPfu DNA Polymerase.
• In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of the non-mutated parental plasmid with DMT Chemically Competent Cell, so as to efficiently select mutant clones.

Advantages of using the Fast Mutagenesis System

• Similarly to the QuickChange site-directed mutagenesis strategy, mutation sites on both primers to improve the mutation efficiency.
• Partially overlapping primers for exponential DNA amplification.
• TransStart FastPfu DNA Polymerase has an extension rate of 4 kb/min and fidelity is 54 times higher than EasyTaq DNA Polymerase.
• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency.
• DMT competent cells are included.

Manual and Datasheet:

FM111 Fast Mutagenesis System

• Read CivicBio’s protocol for the Fast Mutagenesis Strategy
• Read all other site-directed mutagenesis strategies.