FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit - E-EL-0009

FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit

The FA/VB9 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with FA/VB9. During the reaction, Universal FA/VB9 in the sample or standard competes with a fixed amount of FA/VB9 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal FA/VB9. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal FA/VB9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: FA/VB9

Abbreviation : Folate, Vitamin M, Vitamin Bc , Folacin, Pteroyl-L-Glutamic Acid, Pteroyl-L-Glutamate

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 7,5

Detection Range : 12.5-800 ng/mL

More information about this FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit from Elabscience