DMT Enzyme ( Dpn I ) - GD111
Buy 1000 to 1800 units of DMT (DpnI) and receive an instant 7.5% discount.
Buy 2000 units or more and enjoy an instant 15% discount.
Buy 4000 units of DpnI (20 packs) or more to obtain an instant 25% discount!
$72.00 CAD
0 in stock (expect 7-21 days for delivery)
DMT Enzyme
DMT, also known as DpnI, cuts the sequence mGATC (G is methylated) but does not cut the sequence GATC (G is not methylated). This enzyme cuts DNA prepared from most commonly used E.coli strains (dam+ strain), but does not cut PCR products.
Source
An E.coli strain that carries the cloned DMT enzyme gene from Diplococcus pneumoniae.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 μg of pBR322 DNA (prepared from dam+ strain) in 50 μl of reaction mixture in 1 hour at 37°C.
Quality Control
Functional absence of endonucleases and exonucleases activities
Applications
In vitro site-directed mutagenesis, digestion of methylated DNA.
Concentration
10 units/μl
Storage
at -20 °C for two years
Manual and datasheet
GD111 DMT Enzyme (DpnI)
Additional information
| Format | 200 u |
|---|---|
| Supplier |
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